top_email.jpg 一般シーケンス / premix / その他 NGS / BI



サンプル条件

  • Sample Quality Check(QC)の際にsampleを2-3ulを使用します。
  • Sample volumeは余裕をもってご準備下さい。
  • 下記サンプル基準は2019年7月29日時点の基準となります。

Illumina DNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type Library Kit Type Total Amount Conc. DIN etc
Amplicon DNA library Metagenome Amplicon gDNA > 0.1ng/ul >10ul
Target amplicon DNA Amplicon > 0.1ng/ul >10ul
Targeted DNA library SureSelect V6 gDNA 1.000ug >50ng/ul
SureSelect V6 (FFPE) gDNA 0.200ug >10ng/ul
SureSelect V5 gDNA 1.000ug >50ng/ul
SureSelect V5 (FFPE) gDNA 0.200ug >10ng/ul
SureSelect Mouse gDNA 1.000ug >50ng/ul
Whole Genome library TruSeq Nano DNA (350) gDNA 0.100ug >10ng/ul
TruSeq Nano DNA (550) gDNA 0.200ug >20ng/ul
TruSeq Nano DNA (LMW) Amplicon etc. 0.100ug
TruSeq DNA PCR Free (350) gDNA 1.000ug >20ng/ul
TruSeq DNA PCR Free (550) gDNA 2.000ug >20ng/ul
Nextera DNA XT gDNA 0.001ug >0.2ng/ul
Accel Methyl-Seq DNA library gDNA 0.200ug >10ng/ul
TruSeq ChIP-seq library gDNA 0.010ug >1ng/ul
Chromium Genome library gDNA 1.000ug >50ng/ul >7.0
Nextera mate pair library (gel plus) gDNA 4.000ug >100ng/ul


PacBio DNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type Library Kit Type Total Amount Conc. DIN etc
PacBio RSII Library 20kb SMRTbell TPK gDNA 8.000ug >50ng/ul
10kb SMRTbell TPK gDNA 6.000ug >50ng/ul
PacBio Amplicon Library Construction (1kb to 5 kb) Amplicon etc. 1.000ug >50ng/ul
PacBio Sequel Library 20kb SMRTbell Express gDNA 8.000ug >50ng/ul


Illumina RNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください

Library Type Library Kit Type Total Amount Conc. RIN rRNA ratio DV200 etc
mRNA library TruSeq stranded mRNA mRNA 0.100ug >10ng/ul
TruSeq stranded mRNA Total RNA 1.000ug >20ng/ul 7 1
TruSeq stranded mRNA exosomal RNA 0.010ug
SMARTer Ultra low input RNA library Total RNA 0.010ug >2ng/ul 7 1
SMARTer Stranded RNA library Total RNA 0.050ug >2ng/ul 7 1
TruSeq RNA Access FFPE RNA 0.100ug 50%
Small RNA library TruSeq Small RNA library Total RNA 3.000ug 7 1
TruSeq Small RNA library exosomal RNA 0.010ug
TruSeq Small RNA library small RNA 0.100ug
SMARTer Small RNA library Total RNA 0.050ug >10ng/ul 7 1
SMARTer Small RNA library small RNA 0.010ug
SMARTer Small RNA library exosomal RNA 0.010ug
NEBNext Small RNA library Total RNA 3.000ug 7 1
NEBNext Small RNA library small RNA 0.100ug
NEBNext Small RNA library exosomal RNA 0.010ug
Total RNA library TruSeq Stranded Total RNA with Ribo-Zero H/M/R Total RNA 0.500ug >20ng/ul 7 1
TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold Total RNA 0.500ug >20ng/ul 7 1
TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin Total RNA 0.500ug >20ng/ul 7 1
TruSeq Stranded Total RNA with Ribo-Zero Plant Total RNA 0.500ug >20ng/ul 7 1
TruSeq Stranded Total RNA (NEB Microbe) Total RNA 1.000ug 7 1


PacBio RNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください。

Library Type Library Kit Type Total Amount Conc. RIN rRNA ratio DV200 etc
PacBio RSII Library PacBio RSII Iso-Seq Library Construction Total RNA 2.000ug >100ng/ul 7 1
Sequel PacBio Library PacBio Sequel Iso-Seq Library Construction Total RNA 2.000ug >100ng/ul 7 1
PacBio Sequel Iso-Seq Library Construction (+4kb) Total RNA 3.000ug >100ng/ul 7 1


Library Sequence (Run Only)

調製済みライブラリーをご送付いただく際は下記の濃度、液量をご準備ください。

Platform Library Type Type Conc
(nM)
Conc
(ng/ul)
Size(bp)
From
Size(bp)
To
etc
NovaSeq NGS library (Ready to Run) Library 10 at least 20ul
MiSeq NGS library (Ready to Run) Library 5 at least 20ul
HiSeq2500 NGS library (Ready to Run) Library 5 at least 20ul
HiSeq4000 NGS library (Ready to Run) Library 5 at least 20ul
HiSeqX NGS library (Ready to Run) Library 5 at least 20ul


HOME | 会社紹介 | 受託サービス | お問い合わせ | サイトポリシー | プライバシーポリシー | マイページ | 管理者ログイン
本社・ゲノムセンター 〒606-8501 京都府京都市左京区吉田本町36-1
        京都大学国際科学イノベーション棟3F

TEL 075-746-2773 ㈹   FAX  075-746-2775
東京営業所 〒150-0032 東京都渋谷区鶯谷町2-8 ユニオンスクエアビル4F
TEL 03-5784-4350 ㈹   FAX 03-5784-4351
MACROGEN   USA
MACROGEN   APAC
MACROGEN   EUROPE
MACROGEN   KOREA
Copyright 2006-2017 (C) Macrogen Japan Corp. All rights reserved