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サンプル条件

  • Sample Quality Check(QC)の際にsampleを2-3ulを使用します。
  • Sample volumeは余裕をもってご準備下さい。
  • 下記サンプル基準は2020年11月30日時点の基準となります。

Illumina DNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type Library Kit Type Total Amount Conc. DIN etc
Amplicon DNA library Metagenome Amplicon gDNA > 0.1ng/ul >10ul
Target amplicon DNA Amplicon > 0.1ng/ul >10ul
Targeted DNA library SureSelect V6 gDNA 1.000ug >20ng/ul
SureSelect V6 (FFPE) gDNA 0.200ug >10ng/ul
SureSelect V5 gDNA 1.000ug
SureSelect V5 (FFPE) gDNA 0.200ug
SureSelect Mouse gDNA 1.000ug
Whole Genome library TruSeq Nano DNA (350) gDNA 0.100ug >10ng/ul
TruSeq Nano DNA (550) gDNA 0.200ug >20ng/ul
TruSeq Nano DNA (LMW) Amplicon etc. 0.100ug
TruSeq DNA PCR Free (350) gDNA 1.000ug >20ng/ul
TruSeq DNA PCR Free (550) gDNA 2.000ug >20ng/ul
Nextera DNA XT gDNA 0.001ug
Accel Methyl-Seq DNA library gDNA 0.200ug
TruSeq ChIP-seq library gDNA 0.010ug
Nextera mate pair library (gel plus) gDNA 4.000ug


PacBio DNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type Library Kit Type Total Amount Conc. DIN etc
PacBio RSII Library 20kb SMRTbell TPK gDNA 8.000ug >9.0
10kb SMRTbell TPK gDNA 6.000ug >9.0
PacBio Amplicon Library Construction (1kb to 5 kb) Amplicon etc. 1.000ug
PacBio Sequel Library 20kb SMRTbell Express gDNA 8.000ug >9.0
PacBio Amplicon Library Construction (1kb to 5 kb) Amplicon etc. 1.000ug
PacBio Sequel Microbial Library Construction gDNA 3.000ug >9.0
PacBio SequelⅡ Library Large insert (>15kb size-selection cut off) gDNA 8.000ug >9.0
HiFi (10 kb - 25 kb) gDNA 16.000ug >9.0


Illumina RNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください

Library Type Library Kit Type Total Amount Conc. RIN rRNA ratio DV200 etc
mRNA library TruSeq stranded mRNA mRNA 0.100ug >10ng/ul
TruSeq stranded mRNA Total RNA 1.000ug >20ng/ul 7 1
TruSeq stranded mRNA exosomal RNA 0.010ug
SMARTer Ultra low input RNA library Total RNA 0.010ug 7 1
SMARTer Stranded RNA library Total RNA 0.050ug 7 1
TruSeq RNA Access FFPE RNA 0.100ug 50%
Small RNA library TruSeq Small RNA library Total RNA 3.000ug 7 1
TruSeq Small RNA library exosomal RNA 0.010ug
TruSeq Small RNA library small RNA 0.100ug
SMARTer Small RNA library Total RNA 0.050ug 7 1
SMARTer Small RNA library small RNA 0.010ug
SMARTer Small RNA library exosomal RNA 0.010ug
NEBNext Small RNA library Total RNA 3.000ug 7 1
NEBNext Small RNA library small RNA 0.100ug
NEBNext Small RNA library exosomal RNA 0.010ug
Total RNA library TruSeq Stranded Total RNA with Ribo-Zero H/M/R Total RNA 0.500ug 7 1
TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold Total RNA 0.500ug 7 1
TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin Total RNA 0.500ug 7 1
TruSeq Stranded Total RNA with Ribo-Zero Plant Total RNA 0.500ug 7 1
TruSeq Stranded Total RNA (NEB Microbe) Total RNA 1.000ug 7 1


PacBio RNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください。

Library Type Library Kit Type Total Amount RIN rRNA ratio DV200 etc
PacBio RSII Library PacBio RSII Iso-Seq Library Construction Total RNA 2.000ug 7 1
Sequel PacBio Library PacBio Sequel Iso-Seq Library Construction Total RNA 0.600ug 7 1
PacBio Sequel Iso-Seq Library Construction (+4kb) Total RNA 3.000ug 7 1


Library Sequence (Run Only)

調製済みライブラリーをご送付いただく際は下記の濃度、液量をご準備ください。

Platform Library Type Type Conc
(nM)
Conc
(ng/ul)
Size(bp)
From
Size(bp)
To
etc
NovaSeq NGS library (Ready to Run) Library 10 at least 20ul
MiSeq NGS library (Ready to Run) Library 5 at least 20ul
HiSeq2500 NGS library (Ready to Run) Library 5 at least 20ul
HiSeq4000 NGS library (Ready to Run) Library 5 at least 20ul
HiSeqX NGS library (Ready to Run) Library 5 at least 20ul


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