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サンプル条件

  • Sample Quality Check(QC)の際にsampleを2-3ulを使用します。
  • Sample volumeは余裕をもってご準備下さい。
  • 下記サンプル基準は2022年6月2日時点の基準です。

Illumina DNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type Library Kit Type Total Amount Conc. DIN etc
Amplicon DNA library Metagenome Amplicon gDNA > 0.1ng/ul >10ul
Target amplicon DNA Amplicon > 0.1ng/ul >10ul
TruSeq Nano DNA (LMW) Amplicon etc. 0.1ug
Targeted DNA library SureSelect V6 gDNA 1.0ug >7.0
SureSelect V6 (FFPE) gDNA 0.2ug
SureSelect V5 gDNA 1.0ug >7.0
SureSelect V5 (FFPE) gDNA 0.2ug
SureSelect Mouse gDNA 1.0ug >7.0
Whole Genome library TruSeq Nano DNA (350) gDNA 0.1ug >7.0
TruSeq Nano DNA (550) gDNA 0.2ug >7.0
TruSeq DNA PCR Free (350) gDNA 1.0ug >7.0
TruSeq DNA PCR Free (550) gDNA 2.0ug >7.0
Nextera DNA XT gDNA 0.001ug >7.0
Accel Methyl-Seq DNA library gDNA 0.2ug >7.0
TruSeq ChIP-seq library gDNA 0.01ug >7.0


PacBio DNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。
Library Type Library Kit Type Total Amount Conc. Avg.Size (BP) etc
PacBio Sequel Library 20kb SMRTbell Express gDNA 8.1ug 20,000
10kb SMRTbell Express gDNA 8.1ug 15,000
PacBio Amplicon Library Construction (1kb to 5 kb) Amplicon etc. 1.000ug
PacBio Sequel Microbial Library Construction gDNA 4.1ug 20,000
PacBio SequelⅡ Library 20kb SMRTbell Express gDNA 8.1ug 20,000
10kb SMRTbell Express gDNA 8.1ug 15,000
HiFi (10 kb - 25 kb) gDNA 8.1ug 25,000


Illumina RNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください

Library Type Library Kit Type Total Amount Conc. RIN rRNA ratio DV200 etc
mRNA library TruSeq stranded mRNA mRNA 0.1ug
TruSeq stranded mRNA Total RNA 1.0ug 7 1
TruSeq stranded mRNA exosomal RNA 0.01ug
SMART-Seq v4 (SMARTer Ultra low input RNA library) Total RNA 0.01ug 7 1
SMARTer Stranded RNA library (PolyA) Total RNA 0.05ug 7 1
TruSeq RNA Access FFPE RNA 0.1ug 50%
Small RNA library TruSeq Small RNA library Total RNA 3.0ug 7 1
TruSeq Small RNA library exosomal RNA 0.01ug
TruSeq Small RNA library small RNA 0.1ug
SMARTer Small RNA library Total RNA 0.05ug 7 1
SMARTer Small RNA library small RNA 0.01ug
SMARTer Small RNA library exosomal RNA 0.01ug
NEBNext Small RNA library Total RNA 1.0ug 7 1
NEBNext Small RNA library small RNA 0.1ug
NEBNext Small RNA library exosomal RNA 0.01ug
Total RNA library TruSeq Stranded Total RNA with Ribo-Zero H/M/R Total RNA 0.5ug 7 1
TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold Total RNA 0.5ug 7 1
TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin Total RNA 0.5ug 7 1
TruSeq Stranded Total RNA with Ribo-Zero Plant Total RNA 0.5ug 7 1
TruSeq Stranded Total RNA (NEB Microbe) Total RNA 0.5ug 7 1
SMARTer Stranded RNA library (RiboZero) Total RNA 0.1ug 7 1


PacBio RNA Library

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください。

Library Type Library Kit Type Total Amount RIN rRNA ratio DV200 etc
Sequel PacBio Library PacBio Sequel Iso-Seq Library Construction Total RNA 0.600ug 7 1
PacBio Sequel Iso-Seq Library Construction (+4kb) Total RNA 3.000ug 7 1


Library Sequence (Run Only)

調製済みライブラリーをご送付いただく際は下記の濃度、液量をご準備ください。

Platform Library Type Type Conc
(nM)
Conc
(ng/ul)
Size(bp)
From
Size(bp)
To
etc
NovaSeq6000 NGS library (Ready to Run) Library 10 at least 20ul
MiSeq NGS library (Ready to Run) Library 5 at least 20ul
HiSeq2500 NGS library (Ready to Run) Library 5 at least 20ul
HiSeqX NGS library (Ready to Run) Library 5 at least 20ul


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